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Enzymatic hydrolysis could be executed making use of possibly just one enzyme or a combination of multiple enzymes like a pre-remedy to liberate bioactive compounds a lot more effectively from pure by-merchandise [11]. Quite a few experiments have demonstrated that making use of proteases like Alcalase, pepsin, and trypsin to hydrolyze delipidated and freeze-dried fish roe can create peptides with antioxidant action, antibacterial Qualities, anticancer Qualities and enzyme inhibitory consequences [two,twelve]. While in the current analyze, a different professional bacterial protease, HT, and also a fungal protease, FP-II were investigated for their capabilities to supply antioxidant fish roe hydrolysates from Gemfish and Hoki roes and Alcalase was made use of along with as being a reference enzyme for comparative purposes.
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